|
KMID : 0364820220580020091
|
|
Korean Journal of Microbiology
2022 Volume.58 No. 2 p.91 ~ p.95
|
|
|
Complete genome sequence of soybean dwarf virus infecting soybean (Glycine max L.)
|
|
Jo Yeon-Hwa
Choi Ho-Seong
Lee Bong-Choon
Hong Jin-Sung
Cho Won-Kyong
|
|
|
Abstract
|
|
|
|
We found a soybean plant displaying severe viral disease symptoms, such as stunting, rugosity, and yellowing. To reveal the viral agent, we extracted total RNA from the leaf sample and prepared a ribosome-deleted library followed by RNA- sequencing. Raw data were de novo assembled, and the assembled contigs were used in a BLASTX search against a viral protein database. We identified four contigs associated with Soybean dwarf virus (SbDV) belonging to the genus Luteovirus in the family Luteoviridae. We obtained the complete genome of SbDV isolate HS with 5,686 nucleotides (nt), encoding five open reading frames (ORFs). The phylogenetic analysis showed that SbDV isolate HS belongs to Group A containing isolates from the USA and the Czech Republic. Here, we report the complete genome sequence of SbDV isolate HS infecting soybean in Korea obtained by RNA-sequencing.
|
|
|
KEYWORD
|
|
|
genome, soybean dwarf virus, soybean, virus
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
|